Despite its advantages, RT-PCR has some limitations:
Cost: The technique can be expensive due to the need for specialized equipment and reagents. Technical Expertise: It requires skilled personnel to perform the test and interpret the results. Contamination Risk: There is a risk of contamination leading to false positives if strict protocols are not followed. Sample Quality: The quality of the sample can affect the accuracy of the test, with degraded RNA leading to false negatives.
