Sample Collection: Biological samples such as swabs, blood, or tissue are collected from patients. RNA Extraction: RNA is extracted from the collected samples. Reverse Transcription: The extracted RNA is converted into cDNA using the enzyme reverse transcriptase. PCR Amplification: The cDNA is amplified using PCR, which involves repeated cycles of denaturation, annealing, and extension. Detection: The amplified DNA is detected and quantified, usually via fluorescence.
