What is Reverse Transcription Polymerase Chain Reaction (RT-PCR)?
Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a laboratory technique that combines reverse transcription of RNA into DNA (complementary DNA or cDNA) and subsequent amplification of specific DNA targets using polymerase chain reaction (PCR). This method is widely used in molecular biology to measure the amount of a specific RNA. It is particularly valuable in epidemiology for the detection and quantification of viral RNA in samples.
