Real-Time PCR, also known as quantitative PCR (qPCR), is a variation of the standard PCR technique. It enables the simultaneous amplification and quantification of DNA. By using fluorescent dyes or probes, Real-Time PCR provides real-time tracking of the amplification process, allowing for the determination of the initial quantity of the target DNA in the sample. This quantitative aspect is particularly valuable in monitoring disease progression and assessing viral load.