PCR offers several advantages, including high sensitivity, specificity, and the ability to detect low quantities of DNA. It is also relatively rapid and can be automated for high-throughput testing. However, there are limitations to consider. PCR requires specialized equipment and technical expertise, which may not be readily available in resource-limited settings. Additionally, contamination can lead to false-positive results, and the technique cannot distinguish between viable and non-viable organisms, which may affect the interpretation of results.
