Several types of neutralization assays are commonly used, each with its specific applications:
1. Plaque Reduction Neutralization Test (PRNT): Measures the reduction in viral plaques formed on a cell monolayer. It's considered a gold standard for quantifying neutralizing antibodies. 2. Microneutralization Assay: Similar to PRNT but performed in a 96-well plate format, allowing for high-throughput screening. 3. Pseudovirus Neutralization Assay: Uses a non-replicating virus that mimics the pathogen of interest, providing a safer alternative for highly virulent pathogens. 4. Focus Reduction Neutralization Assay (FRNT): Similar to PRNT but uses immunostaining to detect infected cells, increasing sensitivity.
