Neutralization assays, while powerful, come with several challenges:
1. Standardization: Variability in assay protocols can lead to inconsistent results, making it difficult to compare studies. 2. Biosafety: Handling live pathogens requires high biosafety levels, especially for dangerous viruses. 3. Technical Expertise: These assays require skilled personnel and sophisticated laboratory equipment. 4. Interpretation: Results can be influenced by factors such as the choice of cell line and the condition of the virus preparation.
