What Are the Steps Involved in In Situ Hybridization?
The ISH process involves several key steps:
Sample Preparation: Tissues or cells are fixed and embedded in a suitable medium, typically paraffin. Sectioning: Thin sections of the tissue are cut and placed on microscope slides. Probe Preparation: Labeled nucleic acid probes (DNA or RNA) are synthesized to complement the target sequence. Hybridization: Probes are applied to the tissue sections and allowed to hybridize with the target nucleic acid sequences under specific conditions. Detection: The labeled probes are detected using various methods, such as fluorescence or chromogenic detection. Analysis: The results are analyzed under a microscope to visualize the distribution and localization of the target sequences.
