What are the Limitations of In Situ Hybridization?
Despite its advantages, ISH has some limitations:
Technical Complexity: The procedure is technically demanding and requires specialized equipment and trained personnel. Sensitivity: While ISH is highly specific, it may not be as sensitive as other molecular techniques like PCR, limiting its ability to detect low-abundance targets. Time-Consuming: The process can be time-consuming, especially when analyzing large numbers of samples.
