What are the Methods Used to Study Gene Regulation?
Several techniques are used to study gene regulation, including:
Chromatin Immunoprecipitation (ChIP): This method allows the identification of DNA regions bound by specific proteins, helping to map regulatory elements. RNA Sequencing (RNA-Seq): This technique measures gene expression levels and can identify differentially expressed genes under various conditions. CRISPR/Cas9: This gene-editing tool can be used to modify regulatory elements and study their effects on gene expression. Epigenetic Profiling: Techniques like bisulfite sequencing and ChIP-Seq can be used to study DNA methylation and histone modifications, which are key regulators of gene expression.