Despite its advantages, single marker IHC has certain limitations: 1. Specificity and Sensitivity: The accuracy of IHC depends on the quality and specificity of the antibodies used. Non-specific binding can lead to false-positive results. 2. Quantification: While IHC provides qualitative data, quantifying the level of antigen expression can be challenging and may require supplementary techniques. 3. Technical Expertise: Performing IHC requires specialized skills and experience to ensure reliable and reproducible results. 4. Tissue Variability: Variations in tissue processing and antigen retrieval methods can affect the consistency of results.
