Despite its advantages, immunofluorescence has some limitations:
- Technical Complexity: The technique requires specialized equipment and expertise, which may not be available in all settings. - Cost: Fluorescent dyes and antibodies can be expensive. - Artifact Formation: Non-specific binding and autofluorescence can lead to artifacts, complicating the interpretation of results. - Sample Preparation: Proper sample preparation is crucial, as poor handling can degrade antigens and affect the accuracy of the results.
