1. Direct Immunofluorescence: This method involves using a single antibody that is directly conjugated to a fluorescent dye. It binds directly to the target antigen in the sample.
2. Indirect Immunofluorescence: This involves using a primary antibody that binds to the target antigen, followed by a secondary antibody that is conjugated to a fluorescent dye. The secondary antibody binds to the primary antibody, amplifying the signal.
