Despite its advantages, mIHC also presents certain challenges:
Technical Complexity: The technique requires specialized equipment and technical expertise for optimal results. Standardization: Standardizing protocols and ensuring reproducibility can be challenging due to variations in antibody performance and tissue processing. Data Analysis: Analyzing the complex data generated by mIHC requires advanced bioinformatics tools and expertise in image analysis. Cost: Although cost-effective in the long run, the initial setup and reagent costs can be high.
