The procedure for pouring plates involves several key steps:
1. Preparation of Media: Specific nutrient media are prepared and sterilized to support the growth of the target microorganism. 2. Pouring the Plates: The liquid medium is poured into sterile petri dishes and left to solidify. 3. Inoculation: The sample containing microorganisms is added to the solidified medium. This can be done either by spreading the sample on the surface or mixing it directly with the liquid medium before it solidifies. 4. Incubation: The inoculated plates are incubated at appropriate temperatures to allow microbial growth. 5. Colony Counting and Identification: Post-incubation, colonies are counted and identified based on their morphological and biochemical characteristics.
