NOAEL is typically determined through a series of steps in experimental settings:
1. Selection of Test Subjects: Test subjects, often animals, are selected and divided into various groups, including a control group and several experimental groups exposed to different doses of the substance. 2. Exposure: These groups are then exposed to the substance over a specific period. 3. Observation and Data Collection: Researchers observe and document any adverse effects, including physiological, biochemical, and behavioral changes. 4. Data Analysis: The data is analyzed to compare the incidence of adverse effects across different groups. The highest dose that does not produce a statistically significant increase in adverse effects compared to the control group is identified as the NOAEL.
