The process begins with the collection of a biological sample, which could be blood, urine, sputum, or tissue. The sample is then placed in a nutrient-rich medium that encourages the growth of any present microorganisms. This medium is incubated under specific conditions to support microbial growth. After a defined incubation period, the cultures are examined for the presence of colonies that indicate microbial growth. Techniques such as Gram staining and biochemical tests are used to identify the microorganisms.
