Preparing broth cultures involves mixing specific nutrients in water to create a liquid medium that supports microbial growth. The composition of the broth can vary depending on the microorganism being cultured. Once prepared, the broth medium is sterilized to prevent contamination. Clinical samples, such as blood, urine, or swabs, are then inoculated into the broth. The inoculated broth is incubated at a suitable temperature to promote microbial growth, and periodic observations are made to monitor growth patterns.
