Traditionally, antitoxins are produced by immunizing animals, such as horses, with small, non-lethal doses of a toxin. Over time, these animals develop high levels of antibodies against the toxin. Blood is then collected from the immunized animals, and the antitoxin is extracted and purified for medical use. Advances in biotechnology are also enabling the production of recombinant antitoxins, which can be produced in vitro.
