What are the limitations of using these stains in epidemiological research?
While useful, these stains have limitations. Bromthymol Blue is not specific to any particular pathogen and can be influenced by various environmental factors, potentially leading to false positives or negatives. Acid Fuchsin requires precise application techniques, and its effectiveness can vary based on the tissue type and the fixative used. Moreover, the interpretation of stained samples often requires expert training, which can be a limiting factor in resource-constrained settings.
