In epidemiology, these compounds are primarily used in laboratory settings for the identification and study of pathogens. Bromthymol Blue is often employed in culture media to detect metabolic activities of microorganisms. For example, it can indicate the presence of acid-producing bacteria in a sample by changing color. Acid Fuchsin, on the other hand, is utilized to highlight specific structures in tissue samples and microorganisms, aiding in the diagnosis of diseases and identifying pathogen morphology.