While qPCR is a powerful tool, it has limitations: - Cost: The equipment and reagents required for qPCR can be expensive. - Technical Expertise: Performing qPCR requires specialized training and technical expertise. - Sample Quality: The accuracy of qPCR depends on the quality of the sample and the DNA/RNA extraction process. - Inhibition: Certain substances in the sample can inhibit the qPCR reaction, leading to false-negative results.
