What are the Key Methodological Challenges in EWAS?
Several methodological challenges complicate EWAS:
1. Heterogeneity: Tissue-specific and cell-type-specific epigenetic patterns can vary, making it challenging to interpret results from mixed-tissue samples. 2. Confounding Factors: Factors like age, gender, and smoking can confound results, necessitating complex statistical adjustments. 3. Data Quality: High-throughput technologies used in EWAS, such as bisulfite sequencing, require rigorous quality control to minimize technical noise. 4. Replication: Replicating findings across independent cohorts is essential but often difficult due to variations in study design and population characteristics.
