1. Sample Preparation: Biological samples such as blood, tissue, or cells are collected and prepared for analysis. 2. Protein Separation: Techniques like mass spectrometry or two-dimensional gel electrophoresis are used to separate proteins based on their properties. 3. Protein Identification and Quantification: Advanced methods, including tandem mass spectrometry (MS/MS) and liquid chromatography, help identify and quantify proteins. 4. Data Analysis: Bioinformatics tools and software are employed to analyze the data, identify patterns, and draw conclusions.
