Ergosterol is typically measured using chromatographic techniques such as high-performance liquid chromatography (HPLC) or gas chromatography-mass spectrometry (GC-MS). These methods are sensitive and accurate, allowing for the detection of ergosterol in complex matrices like soil, air, and biological tissues. The quantification of ergosterol can provide a proxy for fungal biomass, helping epidemiologists assess the extent of fungal contamination and exposure.
