The HPLC process involves several key components and steps:
Mobile Phase: The solvent or mixture of solvents that carries the sample through the column. Stationary Phase: The solid adsorbent material inside the column that separates the sample components based on their interactions with it. Sample Injection: The process of introducing the sample mixture into the mobile phase. Detection: Various detectors, such as UV-Vis, fluorescence, and mass spectrometry, are used to identify and quantify the separated components.
