The study of virulence factors involves various techniques and approaches:
1. Molecular Biology: Techniques like PCR and gene sequencing are used to identify genes responsible for virulence. 2. Biochemical Methods: These methods help in characterizing the biochemical properties of virulence factors. 3. Animal Models: Pathogens are studied in animal models to understand the role of virulence factors in disease progression. 4. Epidemiological Studies: Case-control and cohort studies help in understanding the impact of virulence factors on disease spread and severity.