mutation detection

How are Mutations Detected?

There are various methods used for mutation detection, including:
1. Polymerase Chain Reaction (PCR): A widely used technique to amplify small segments of DNA, making it easier to identify specific mutations.
2. Next-Generation Sequencing (NGS): A high-throughput method that allows for the rapid sequencing of large amounts of DNA, providing a comprehensive view of the genetic makeup of pathogens.
3. CRISPR-Cas9: A cutting-edge technology that can be used to accurately identify and edit specific genetic sequences, making it useful for pinpointing mutations.
4. Sanger Sequencing: An older but still reliable method for sequencing short strands of DNA, often used to confirm mutations identified by other techniques.

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