The development of in vitro models involves several steps:
1. Cell Culture: Cells are isolated from tissues and grown under controlled conditions. These cells can be primary cells, immortalized cell lines, or stem cells. 2. Model Design: The experimental setup is designed to mimic the in vivo environment as closely as possible. This includes the use of appropriate growth media, substrates, and environmental conditions. 3. Validation: The model is validated by comparing its responses to known in vivo outcomes. This step ensures that the model accurately represents the biological system it aims to replicate.
