Cytotoxicity tests involve a series of steps: 1. Cell Culture: Cells are cultured in a laboratory setting under controlled conditions. 2. Exposure: The cells are exposed to the test substance at varying concentrations. 3. Assessment: Several methods are used to assess cytotoxicity, including: - MTT Assay: Measures cell viability based on metabolic activity. - LDH Assay: Detects cell membrane damage through the release of lactate dehydrogenase. - Flow Cytometry: Analyzes cell death and apoptosis by measuring cell size and granularity.
