The process generally involves the following steps: 1. Sample Collection: Proper collection and handling of clinical specimens are critical. 2. Inoculation: The sample is inoculated onto or into the culture media using aseptic techniques. 3. Incubation: The inoculated media are incubated at optimal conditions for the growth of the target microorganisms. 4. Observation and Analysis: Colonies are observed for growth patterns, color, and morphology. Further tests may include Gram staining, biochemical tests, and molecular techniques.
