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third generation sequencing
What is Third Generation Sequencing?
Third generation sequencing (TGS) refers to advanced sequencing technologies that enable the direct reading of long DNA or RNA sequences in real-time. Unlike
first
and
second generation sequencing
, TGS can sequence single molecules without the need for amplification, leading to more accurate and faster results. Key technologies in TGS include
Pacific Biosciences' Single Molecule Real-Time (SMRT)
sequencing and
Oxford Nanopore Technologies (ONT)
sequencing.
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