The TST has several limitations: - False Positives: Can occur in individuals who have been vaccinated with the BCG vaccine or exposed to non-tuberculous mycobacteria. - False Negatives: Can occur in individuals with weakened immune systems, very young children, or those recently infected where the immune response has not yet developed. - Booster Effect: Repeat testing can sometimes cause a boosted reaction in individuals previously sensitized to TB antigens.
