Several techniques are essential in proteomics, including:
1. Mass Spectrometry (MS): This technique identifies and quantifies proteins in complex mixtures by measuring the mass-to-charge ratio of ionized particles. 2. Two-Dimensional Gel Electrophoresis (2-DE): Used to separate proteins based on their isoelectric point and molecular weight. 3. Protein Microarrays: Allow high-throughput screening of protein-protein interactions, antibody-antigen interactions, and enzyme-substrate interactions. 4. Bioinformatics Tools: Software and databases are crucial for analyzing proteomic data, identifying proteins, and understanding their functions and interactions.
