Since its inception, PulseNet has been instrumental in numerous outbreak investigations: - E. coli O157:H7 in 1997: One of the first successes was identifying the source of an E. coli outbreak, leading to a recall of contaminated ground beef. - Salmonella Saintpaul in 2008: PulseNet helped trace the source of a large Salmonella outbreak to contaminated peppers. - Listeria in 2011: The network was crucial in identifying cantaloupes as the source of a deadly Listeria outbreak.