Proper use of pipettes is crucial for ensuring accurate and reproducible results. Here are key steps to follow:
Calibration: Regular calibration is essential to maintain accuracy. Pre-rinsing: Pre-rinse the pipette tip with the solution to prevent volume discrepancies caused by residual liquid. Angle: Hold the pipette at an appropriate angle (usually between 20° and 45°) to avoid air bubbles. Speed: Dispense and withdraw liquids slowly to avoid splashing and ensure precise volume transfer.
