1. Preparation: The virus to be tested is grown in culture, purified, and quantified. 2. Serum Dilution: Serum samples from individuals are diluted serially. 3. Virus-Serum Incubation: The diluted sera are mixed with a known quantity of the virus and incubated to allow any neutralizing antibodies to bind to the virus. 4. Inoculation: The virus-serum mixtures are then added to cell cultures. 5. Plaque Counting: After incubation, cells are fixed and stained to visualize the plaques, which are counted to assess the degree of neutralization.
