Performing a microbial culture involves several steps: 1. Sample Collection: Samples can be collected from various sources such as blood, urine, swabs, or environmental surfaces. 2. Inoculation: The sample is inoculated onto a culture medium, which provides the nutrients necessary for microbial growth. 3. Incubation: The inoculated media are incubated at appropriate temperatures to allow microorganisms to grow. 4. Identification: Once colonies form, they can be identified using biochemical tests, microscopy, and molecular methods.