The procedure is straightforward and involves the following steps:
Collect the sample: Skin scrapings, hair, or nail clippings are obtained from the affected area. Place the sample on a glass slide. Add a few drops of 10-20% KOH solution to the sample. Cover the sample with a coverslip. Heat the slide gently to accelerate the action of KOH, if needed. Examine the slide under a microscope for the presence of fungal elements.