AST can be performed using various methods, including: 1. Disk Diffusion: This method involves placing antibiotic-impregnated disks on an agar plate inoculated with the bacteria. The zone of inhibition is measured to determine susceptibility. 2. Broth Dilution: This technique determines the minimum inhibitory concentration (MIC) of an antibiotic by exposing bacteria to different concentrations of the drug in a broth medium. 3. E-test: A combination of both disk diffusion and broth dilution, the E-test uses a gradient strip to determine the MIC.
