The process of sandwich ELISA typically involves the following steps:
Coating: A capture antibody specific to the target antigen is coated on a microplate. Blocking: Non-specific binding sites are blocked to prevent background noise. Sample Addition: The sample containing the antigen is added and allowed to bind to the capture antibody. Detection: A detection antibody, conjugated with an enzyme, is added to bind to a different epitope on the antigen. Substrate Addition: A substrate is added, which reacts with the enzyme to produce a measurable signal, usually colorimetric.
