The process of RNA-Seq involves several key steps: 1. Isolation of RNA: Extracting RNA from the cells or tissues of interest. 2. Library Preparation: Converting RNA into cDNA and adding adapters for sequencing. 3. Sequencing: Using next-generation sequencing (NGS) platforms to sequence the cDNA. 4. Data Analysis: Aligning the sequencing reads to a reference genome and quantifying gene expression.
