1. Identifying the Main Research Question: The first step is to clearly define the main research question of the broader study. This provides the context for the subplots.
2. Formulating Specific Objectives: Each subplot should have its own specific objectives that align with the main research question. These objectives should be measurable and achievable.
3. Selecting Relevant Variables: Researchers must identify which variables are most relevant to the subplot. These could include demographic factors, environmental exposures, or genetic markers.
4. Choosing the Appropriate Study Design: Depending on the objectives and variables, researchers might use different study designs for each subplot. For instance, a case-control study might be suitable for examining risk factors, while a cohort study could be better for studying the progression of a disease.
